The sexual harassment scandal that engulfed Fox News last year and led to the ouster of its chairman, Roger Ailes, continued to batter the network on Monday, as a new lawsuit described unwanted sexual advances by Mr. Ailes and two major advertisers pulled their spots from the show of its top-rated host, Bill O’Reilly.

Mercedes-Benz and Hyundai said they were withdrawing their ads from Mr. O’Reilly’s prime-time show, “The O’Reilly Factor,” after The New York Times published an investigation this weekend that found five women who made allegations of sexual harassment or inappropriate behavior against him. Those five women received settlements totaling about $13 million, The Times reported.

Together, the developments portray a network buffeted by allegations on multiple fronts, even as it draws record ratings with programming supportive of President Trump. Staff members remain anxious, some said on Monday, over questions about its workplace culture and its priorities.

If more advertisers abandon Mr. O’Reilly’s show, it will be a blow to Fox News, which provides billions of dollars in revenue each year to its parent company, 21st Century Fox. Mr. O’Reilly has long been the pugnacious face of a prime-time lineup that sets the tone for conservative commentary. His show attracts almost four million viewers a night, and from 2014 through 2016 it generated more than $446 million in advertising revenue, according to the research firm Kantar Media.

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“Given the importance of women in every aspect of our business, we don’t feel this is a good environment in which to advertise our products right now,” Donna Boland, the manager of corporate communications for Mercedes-Benz, wrote in an email. Mercedes-Benz has spent an estimated $1.9 million in ads on “The O’Reilly Factor” in the last year, according to iSpot.tv, the TV ad analytics firm.

Hyundai cited “the recent and disturbing allegations” in announcing that it was removing its ads from Mr. O’Reilly’s show. “We had upcoming advertising spots on the show, but are reallocating them,” Hyundai said in an emailed statement.

“As a company, we seek to partner with companies and programming that share our values of inclusion and diversity,” the statement said. “We will continue to monitor and evaluate the situation as we plan future advertising decisions.”

Despite Mr. O’Reilly’s history of settlements and the series of allegations against him, the company has extended his contract, which was set to expire this year, according to people familiar with the matter. Mr. O’Reilly makes about $18 million per year. When the company extended the contract, it knew of multiple settlements that had been reached with women who had complained about his behavior.

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The company says it has discussed the issue with Mr. O’Reilly. It believes his new contract gives it more leverage over him regarding his behavior, according to two people familiar with the matter. Mr. O’Reilly has said that the allegations are without merit. He did not address the controversy on his show Monday night.

Earlier on Monday, Julie Roginsky, a current Fox News contributor, filed a lawsuit against Mr. Ailes, Fox News and Bill Shine, the network’s co-president, asserting that she faced retaliation for rebuffing Mr. Ailes’s sexual advances and for refusing to disparage Gretchen Carlson, the former Fox News host who sued Mr. Ailes last summer.

And a former regular guest on Mr. O’Reilly’s program, Wendy Walsh, who had recounted her allegations against him to The Times, held a news conference with her lawyer to discuss those claims and to call for an independent inquiry into sexual harassment at the network.

Also, the United States attorney’s office in Manhattan is investigating Fox News, including how it structured settlements.

On Monday, Fox News moved to contain the fallout from the weekend’s developments, urging its employees in an internal memo to report inappropriate behavior to the human resources department or other network executives. “Particularly in light of some of the accounts published over the last few days, I wanted to re-emphasize the message we have been conveying at our training sessions for several months,” said Kevin Lord, the network’s new head of human resources, who was hired in the aftermath of the Ailes scandal.